Study Of Stability And Anti-Inflammatory Action Of Naringin Loaded ß-Lactoglobulin Nanoparticles

Abstract

In this study, the effect of naringin loaded ß-lactoglobulin nanoparticles on stability of naringin and its anti-inflammatory action were studied. When exploring the effect of adding β-lactoglobulin nanoparticles, 0.01g β-lactoglobulin nanoparticles was evaluated for stability by total phenolic content and the total antioxidant activity. The total phenolic content in the test samples was determined quantitatively using Folin-Ciocalteu reagent method. The antioxidant action of the synthesized compounds was determined using 2,2-Diphenyl-1-picrylhydrazyl  (DPPH) radical scavenging assay. The anti-inflammatory activity of the test samples of naringin and naringin ß-Lactoglobulin nanoparticles was determined by albumin denaturation assay and the antiprotease activity determination assay. The total phenolic content increased on addition of ß-lactoglobulin and preparation of nanoparticles further improved the total phenolic content suggesting higher heat stability of naringin when formulated as ß-lactoglobulin nanoparticles. It was also witnessed that the formulation of ß-Lg nanoparticles loaded with naringin improved the antioxidant activity of naringin suggesting improved stability of the encapsulated naringin. The naringin loaded ß-lg nanoparticles exhibited higher anti-inflammatory action in comparison to pure naringin in both the assay methods. The improved stability of naringin in nanoparticles may be an attributing factor for the improved activity.