Simultaneous Estimation Of Azithromycin, Fluconazole And Ornidazole In Combined Dosage Form Using Hptlc Method

Abstract

A rapid, stable high-performance thin-layer chromatography (HPTLC) method was created and validated for the simultaneous quantification of azithromycin (AZM), fluconazole (FLZ), and ornedizole (OZ) in Combi-kit amounts from tablets. The stationary phase was made up of HPTLC silica gel 60 F254 plates, while the mobile phase was a combination of toluene, methanol, and 1,4-dioxane in a volume ratio of 2:2:6. A CAMAG TLC Scanner 3 was used to perform densitometric scanning at a wavelength of 210 nm. The validation of the created technique adhered to the standards established by the International Council for Harmonization. The RF values of AZM, FLZ, and OZ were 0.202, 0.382, and 0.522, respectively. In this case, the statistical tests previously described for determining the suitability of the simple linear regression model are applied to our models AZM (y = 4.506x + 2189.4), FLZ (y = 4.506x + 2189.4), and OZ (y = 4.8969x + 240.53). LOD= 3s/S and LOQ = 10s/S (2) Where s is the standard deviation of y-intercept and S is the slope of the calibration curve. The LOD and LOQ were found to be 0.0467and 0.141 g/zone, respectively. These three drugs can be routinely analyzed in their pharmaceutical dosage form using these methods. Results for analysis of both methods were tested and validated for various parameters according to ICH guidelines.