Evaluation of Antimicrobial efficacy of Chrysopogon zizanoides on Staphylococcus aureus, Escherichia coli and candida albicans -an in vitro study

Abstract

Aim: To Compare and analyze the antimicrobial efficacy of  Crude,Ethanolic and aqueous extract of  Chrysopogon  zizanioides, on Staphylococcus aureus, escherichia coli and candida albicans .

Materials and methods : The Selected herb for our research is Vetiver (Chrysopogon zizanioides). Undry  Plant materials were processed and homogenized to fine powder, filtered through Whattman filter paper no.1 and subjected  for antimicrobial sensitivity(crude extract). Then slowly heated 10 grams of dried powder was dissolved in distilled water for six hours, filtered through eight layers of muslin cloth, centrifuged at 5000 rpm for 15 min after which the supernatant was collected and autoclaved at 121ºC and 15 lbs pressure before storing at 4ºC(aqueous extract). The inal extract will be concentrated by distilling off the solvent using a rotary evaporator. Finally, 2 ml of stored plant extracts are dissolved in 1L of Dimethyl sulfoxide 3(organic solvents based xtracts-Ethanol) and Sterile Distilled water (aqueous extracts) to be tested for Antimicrobial efficacy.

Result: It was found that the ethanolic extract had the maximum antimicrobial activity when Compared to Crude ad aqueous extracts against the tested microorganisms. The maximum zone of inhibition against Staphylococcus aureus was noticed on using 10% ethanolic extract, Escherichia coli while using 20% ethanolic extract, and 15% ethanolic extract against Candida albicans.

Conclusion: This investigation of Chrysopogon zizanioides has shown an influence on oral microorganisms and has confirmed its significance especially in the area of influence on tested  Organisms ,Staphylococcus aureus, escherichia coli and candida albicans