Lipid Production Of Yarrowia Lipolytica Using Food Waste Anaerobic Digestate

Abstract

 Microbial lipid production from waste derived material act as an alternative sustainable approach for fossil fuels. In this study, food waste anaerobic digestate (FWAD) was employed as culture medium for oleaginous yeast strain Yarrowialipolytica MTCC 35. To optimize the culture conditions, the initial C/N ratios of the medium were adjusted to 20 using glycerol and after three days C/N ratios of the culture media were adjusted to 40 (C/N-40), 60 (C/N-60), 80 (C/N-80), 100 (C/N-100) and 120 (C/N-120). The following three treatments without C/N ratio adjustment served as controls to reveal the effectiveness of C/N adjustment: (C1) YPD medium, (C2) FWAD without pretreatment and (C3) FWAD with pretreatment C1-YL, C2-YL and C3-YL without any C/N ratio adjustment. Biomass production was the highest in YPD medium (6.73 g/L, dw basis) while the lipid content was the highest (12.53 %, w/w) in FWAD-hydrolysate medium with C/N-80. Results suggested that C/N ratio adjustment of FWAD-hydrolysate medium significantly increase the lipid production of Y. lipolytica and C/N ratio 80 of FWAD-hydrolysate medium was found optimum for high lipid production.