Antibacterial And Antibiofilm Of Klebocin Extracted From Klesiella Pneumoniae On Two Pathogenic Bacteria

Abstract

The main aim of the current study target to evaluate the anti-bacterial and anti-biofilm activity of Klebocin, a type of bacteriocin produced by some bacteria to attack competing bacteria. Bacteriocins are promising alternatives to conventional antibiotics due to their precise targeting and effectiveness against resistant bacteria.

Two hundred clinical samples were collected, 150 of which were confirmed to be infected with Klebsiella pneumoniae using selective culture media and biochemical tests. After isolating Klebocin from the confirmed samples, its biological activity was evaluated against Pseudomonas aeruginosa and Staphylococcus aureus, two clinically significant pathogenic microorganisms.Antibacterial activity was measured using a method known as agar-well diffusion, with Klebocin showing a concentration-dependent inhibitory effect. At the highest concentration tested (0.01 mg/ml), the average diameter of the inhibition zones was 16.2 mm against Pseudomonas aeruginosa and 16.25 mm against Staphylococcus aureus, indicating strong antimicrobial activity. In contrast, there was no activity observed in the control group (0 mg/ml).Biofilm inhibition assays dependent on using microplates also showed that Klebocin significantly decreased biofilm formation in both bacterial species, with the highest inhibition rate at 0.01 mg/ml.

Current results suggest that Klebocin holds promise as an alternative therapeutic option against antibiotic-resistant bacteria, given its potent antimicrobial and antibiofilm properties.