Optimization Of Lipase Synthesis By Pseudomonas Aeruginosa Strain Av311222

Abstract

Lipases are commonly found in nature, breaking the fat-generating glycerol and free fatty acid (FFA) functioning as biocatalyst. Lipases in general are synthesized by animals and microbes. However, industrially the microbes are exploited for the synthesis of lipase. Which depends on many factors and the difference among strain of the microbe is also reported. This study analysedifferent conditions for extracellular lipase production by a bacterial strain isolated from lipid-rich fish market soil. The investigated isolate was identified as P. aeruginosa strain AV311222 by analysing 16S rRNA. The objective of the present study is to determine the activity of lipase under various physiochemical conditions, i.e. temperature, incubation time, inoculum size, substrate, carbon source, organic/inorganic nitrogen sources, surfactant and metal ions. It is noticed that the maximum lipase production was at the medium temperature range of 30℃-42℃, with the pH 6.0, 1% of inoculum size and incubated up to 24 hours. The maximum lipase activity was recorded at the optimized factors containing lactose (1%), meat extract (1%), ammonium dihydrogen phosphate (1%), calcium chloride (1%), Tween 80 (1%) and 1% of palm oil respectively. The findings revealed that the combined approaches enabled rapid and cost effective lipase production.