Molecular Detection Of Virulence And Antibiotics Resistance Gene Of Acinetobacter Baumannii Isolated From Burned Wound Patients

Abstract

Background: Acinetobacter baumannii is identified as one of the most common infections in burn units globally.Objective: The purpose of this study was to assess antibiotic resistance and apply molecular method  to clinical isolates of A. baumannii that were obtained from burned wound. Methods: A total of 150 specimens(burned wound swab) were collected from patients who admit in burns units throughout the period of December 2023 to the Februry 2024. The collected specimens were streaked directly on culture media incubated for 24 hrs at 37ºC. All bacteria isolates were examined by confirm by vitik 2 system. Antibiotic susceptibility test was prepared by vitik 2 system. Acinetobacter baumannii isolates was performed by using Polymerase chain reaction to detect blaoxa 51 Bap ,OmpA, CsuE and PgaAgenes.Results: Flame were higher in females,while Electric was higher in  males. Burn surface area  ˂ 15% and 15 -  25%  were higher in males. Second / degree burns was higher percentage injury from other  degree of burn. Patients injury by flame was more infected with A. baumannii  bacteria by 80 (53.3%). A total of 30 isolates of A. baumannii have been discovered. A PCR was conducted on 15 Multi-Drug Resistant (MDR),(XDR) isolates to identify the presence of genes associated with virulence factors. The results showed that the bap  ompA, pgaA   blaOXA-51-like genes were found to be present in all 15 isolates A. baumannii clinical, while CsuE pili was 0%. Conclusions: Our findings revealed a correlation between genes of antimicrobial resistance and significant biofilm formation.