Fimbrial Detection In Escherichia Coli Isolated From Different Clinical Samples In Kirkuk City

Abstract

Background: Escherichia coli has the ability to cause a range of infections, both in the intestines and in other parts of the body, which can vary in severity from moderate to life-threatening. The degree of infection severity is influenced by numerous virulence genes, including those that encode adhesion molecules such as fimbriae. These molecules aid in the invasion and colonization of epithelial cells.Objective: To detect the presence of type 1, P, S, and F1C fimbriae in different clinical samples. Method: A total of 200 clinical samples (Urine, wound, sputum, and burn) were collected. The samples were diagnosed through culturing on differential and selective media, Biochemical tests and the Vitek 2 system. A hemagglutination test was performed. A polymerase chain reaction was performed for molecular detection of fimbrial genes.

Results: 40 E.coliisolates were obtained (28 from urine, 8 from wound, 2 from sputum and burn, respectively). The highest isolation rate was in females (75%) than in males (25%), with a higher percentage (42.5%) in the age group (17-36) years. For the hemagglutination test (52.5%) of the total isolates were positive, and (47.5%) were negative, (66.6%) were mannose resistant (MRHA), and (33.3%) were mannose sensitive (MSHA). Polymerase chain reaction revealed the percentage of detection was (90%) for fimH gene: (87.5%) from urine, (87.5%) from wound, (100%) from burns, and (100%) from sputum, (30%) for papC gene: (37.5%) from urine, (25%) from wound, (50%) from sputum, (10%) for sfaS gene: (12.5%) from urine, and (12.5%) from wound, (5%) for focG gene: (50%) of sputum sample. Conclusion: The study concluded that Type 1, P, and S fimbriae were observed in urine and wound samples. In sputum samples, type 1, P, and F1C fimbriae were detected, while in burn samples, only type 1 fimbriae was detected.