Green Synthesis Of Titanium Nanoparticle From Leaf Extract Of Prosopis Cineraria And Cytotoxicity Analysis

Abstract

The current research goal was to successfully bio-synthesize titanium NPs from the leaf extracts of Prosopis cineraria. Alkaloids, saponins, flavonoids, tannins, steroids, phenols, carbohydrates, and proteins were found to be the phytochemicals in the leaf at quantities of 268.5, 26.5, 61.7, 27.7, 1.393, 32.1, 7.9, and 1.98 mg/ml during the study. The development of titanium nanoparticle was validated by utilizing UV-Visible spectroscopy showing a peak range of 200-400 nm. The morphology of nanoparticle was studied utilizing TEM analysis which showed a size range of titanium NPs from 13.49 to 23.07 nm at a scale of 200 nm. The FTIR spectrum showed multiple peaks at 3401.46 cm¹ (aromatic primary amine), 3326.69 cm¹ (imino compound, =N-H stretch), 1634.81 cm¹ (amide group) and 686.83 cm¹ (aromatic C-H out-of-plane bend), while the peaks between 666.37cm¹ to 619.26 cm¹ represents the alkyne C-H band, respectively. During the MTT assay, the lowest concentration tested, i.e. 0.125 mg/mL) yielded the highest cell viability at 95.65%. Based on the findings, it can be inferred that the created NPs have the potential to demonstrate various antimicrobial, antioxidant, and pharmacological properties, making them applicable in the biomedicine, environmental pollution, remediation, and healthcare industries. Prosopis cineraria leaf extract is more suitable for nanoparticle synthesis due to its richer phytochemical content, greater efficiency in nanoparticle stabilization, and enhanced biological activity, making it superior to pods, bark, or stem.