In Vitro Propagation And Conservation Strategies For A Medicinal Plant Pterocarpus Marsupium Roxb (C.G.) India

Abstract

Pterocarpus marsupiumRoxb. is a tree species valued for its medicinal properties and high-quality timber. Despite its significance, the species faces threats of extinction due to anthropogenic activities and poor regeneration rates. In vitro multiplication techniques offer a promising approach for its conservation and sustainable use in medicine. This study compared two treatments, MS+0.1BAP and MS+0.5BAP, for their efficacy in shoot multiplication and growth parameters. The results showed that MS+0.5BAP resulted in marginally higher shoot numbers (3.60) and longer shoot lengths (6.26 cm) compared to MS+0.1BAP (3.55 shoots, 5.63 cm). This suggests the superior efficacy of higher BAP concentrations in promoting shoot elongation. Moreover, MS+0.5BAP demonstrated a higher percentage of contamination-free cultures (94.05%) and a slightly elevated survival rate (84.25%) compared to MS+0.1BAP (91.20%, 78.95%, respectively), indicating enhanced culture cleanliness and plantlet viability under higher BAP concentrations. These findings underscore the potential benefits of utilizing MS+0.5BAP for optimizing shoot multiplication and culture success in Pterocarpus marsupium tissue cultures. This study contributes to conservation efforts aimed at preserving the medicinal and ecological significance of P. marsupiumRoxb. species.