An In-Vivo Investigation Of Acute Toxicity Assessment Of Sodium Fluoride To Channa Punctatus (Bloch)

Abstract

In-vivo median lethal and safe concentrations of sodium fluoride in an Indian snake-headed murrel, Channa punctatus (Bloch), weighing 50-60g, has been assessed. Lorke’s method estimated the 96-hour LC50 to be 1385.64 mg/l. As an approximate estimate, the Up-and-Down approach produced a 96-hour-LC50 range of 1500.0 to 1550.0 mg/l. Furthermore, the Behren-Karber, regression, and Finney techniques had 96-hour LC50 values of 1360.0, 1319.78, and 1258.9mg/l, respectively. The cumulative median lethal average of the 96-hour-LC50 dose in Channa punctatus was 1385.64+1525.0+1360.0+1319.78+1258.9 = 1369.86 or 1370 mg/l of sodium fluoride. The dose of sodium fluoride has a significant connection with fish mortality. Sodium fluoride accumulates in aquatic bodies, disrupting the physiological activities of this fish, according to the findings. This fish is more resistant to fluoride toxicity than other fishes. The calculated safety level value indicated that determining an acceptable dose of sodium fluoride for this fish is feasible. Sodium fluoride may even be classified as a slightly/moderately toxic substance to Channa punctatus based on its toxicity range.