Characterisation And Antibacterial Screening Of Indigenous Lactic Acid Strains Against Food Pathogens

Authors

  • Rebzani Ferayale, Aoues Karima, Hammadeche Mohammed Amine, Kaced Amel, Ramdane Sidali, Benrima Atika, Bencherchali Sabrina, Belouzdad Meriem, Abdoun Bouchra Anfal Author

DOI:

https://doi.org/10.64252/taa3nf07

Keywords:

Raw milk; Lactic acid bacteria; Antibacterial activity; MALDI-TOF; Indigenous.

Abstract

In this study, a total of 70 isolates were obtained from raw goat's and cow's milk cultured on MRS and M17 media, 27 of which exhibited the characteristics of lactic acid bacteria. Identification by MALDI-TOF MS revealed that these isolates mainly belonged to the species Enterococcus faecium (55.55%), Pediococcus pentosaceus (22.22%), Lactococcus lactis (7.40%) and Lactobacillus acidophilus (3.70%), while three isolates (11.11%) could not be reliably identified.

Evaluation of antibacterial activity against Escherichia coli ATCC 25922 led to the selection of 11 lactic acid strains. However, tests carried out on Staphylococcus aureus ATCC 25923 reduced this number to five strains, which were selected for further investigation. This work will focus on characterising the antimicrobial metabolites produced, particularly bacteriocins, and evaluating their in situ efficacy in food matrices with a view to potential applications in biopreservation.

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Published

2024-12-15

Issue

Vol. 10 No. 4 (2024)

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Characterisation And Antibacterial Screening Of Indigenous Lactic Acid Strains Against Food Pathogens. (2024). International Journal of Environmental Sciences, 933-940. https://doi.org/10.64252/taa3nf07