In-Vitro Anti Cancer Activity Of N-Benzylidene Aniline

Abstract

 This study aims to describe the  synthesis, characterization   and determination of anticancer activity of N- Benzylidene aniline. Melting point, IR and UV spectral  data were confirm the structure of compound  and anticancer activitiy was characterized by the nature of biological activities.    Colon cancer remains one of the leading causes of cancer-related deaths worldwide. Despite advancements in treatment modalities, there is a continuous demand for novel therapeutic agents with enhanced efficacy and reduced toxicity. This project aims to explore the potential anticancer properties of N-Benzylidene Aniline (NBA) on the HT-29 colon cancer cell line through in-vitro experiments. NBA, a compound with reported pharmacological activities, will be evaluated for its cytotoxic effects, apoptotic induction, cell cycle modulation, and mechanistic insights on HT-29 cells. The findings from this study could provide valuable insights into the development of novel therapeutic strategies for colon cancer treatment.The anticancer activity of the prepared compound was  employed by using the In- vitro MTT Assay  method.