Molecular Detection Of Klebsiella Pneumonia Isolated From Ruminants Secretion In AL Shatrah City, South Iraq

Abstract

In this  current study showed that detection of Klebsiella species from variation infected cattle discharge. 120 samples and swabs were collected divided in to (40 from nasal secretion, 40 from  vaginal discharge  and finally 40  from a milk samples). These sample cultured on different culture media and identified by using cultural characteristic and microscopic appearance. klebsiella pneumonia Identified using ViteKⅡ system and confirm the last diagnosed of those organism by using polymerase chain reaction(PCR)assay (16S rRNA). Polymerase chain reaction used to detected some gene K1,K2(which is one of several genes  responsible for capsule formation), and also blaSHv gene which is one of the genes related with multy drug resistance. Results  explaned that Klebsiella  pneumoniae were isolated in rate  (10%) from nasal discharge, while isolated in rate (10%) from milk samples, on the other hand, vaginal secretion  not appeared positive result for Klebsiella pneumonia.   the result involved  that K1,K2 genes were not detected in all samples of K. pneumonia. Also,  completely isolates of K. pneumoniae harbored  blaSHv gene (100%),  Also the result included that Klebsiella pneumoniae can be consider as major pathogen  responsible  for different  medical case affected bovine in Al-Shatrah district in Thi-Qar province.