In Vitro Evaluation of Antioxidant Activity of Padikara Parpam (PP), L-Ascorbic Acid (LAA), and combination of Padikara Parpam and L-Ascorbic Acid (PPLAA)

Abstract

The antioxidant activity of Padikara Parpam (PP), L-Ascorbic Acid (LAA) and their combination Padikara Parpam and L-Ascorbic Acid (PPLAA) was assessed in this study performing various in vitro tests. The antioxidant activity of these three different test samples PP, LAA and PPLAA was assessed using TAC, DPPH assay, hydrogen peroxide, nitric oxide scavenging activity, estimation of lipid peroxidation using egg yolks, ABTS Radical Cation, superoxide radical, and  deoxyribose radical scavenging activity, β carotene linoleic acid assay, and assay of superoxide dismutase (SOD). The findings indicated that all three compounds demonstrated dose-dependent free radical scavenging, with PPLAA showing the highest activity at 500 µg/mL, particularly against hydrogen peroxide and DPPH (83% inhibition). The compounds effectively inhibited lipid peroxidation, superoxide and nitric oxide radicals, and β-carotene bleaching, demonstrating their capacity to reduce oxidative stress. The antioxidant capability of PP, LAA, and their combination (PPLAA) is demonstrated in this work using in vitro techniques. These findings support the use of them in formulations meant to lessen oxidative stress and associated illnesses that can act as therapeutic agents.