Detection Of Pathogenic Escherichia Coli O157:H7 From Euphrates River And Sheep In AL-Fallujah With Detection Of The Important Virulence Factor Rfb O157 And Flic H7 Genes Which Cause Haemorrhaic Diarrhea In Sheep

Abstract

The aim of the present study to isolation and identification the certain Escherichia coli associated with diarrhea sample of sheep which cause hemorrhagic diarrhea and detection of the important virulence factor Rfb O157 and fliC H7 genes  in E.coli which cause diarrhea in addition to determine the antibiotic resistance of these bacteria. To achieve the goal, 50 of (diarrhea) samples were collected from sheep and 50 sample from water of river using routine bacteriological isolation and identification. The result showed that among 50 small ruminant (sheep) ,31% expressed bacterial isolates,the result of water from Euphrates River in AL- Fallujah ( 50 sample) was 27 positive isolate which equal (54%). A statistical analysis of latex agglutination test revealed no significant differences in the prevalence of E. coli O157:H7 between sources ,Which revealed the E. coli O157:H7  in latex agglutination test produced positive isolation rates The O157 somatic antigen was found in 5(16.1)%  and 6(22.2%) of E. coli isolates from sheep and water respectively and isolates flagellar H7 antigen was evaluated. The frequency of H7 antigen-positive isolates was 2( 6.4%) for sheep isolates, and 1(3.7%) for water isolates. In addition to determine the antibiotic resistance of these bacteria These bacteria showed multi antibiotic resistance which resist for seven antibiotic agents and sensitive for three others were use. The result revealed that all E.coli isolates expressed rfbo157 gene, and showed positive result for fliCH7 gene. from E.coli O157:H7 species pathogenicity and toxin production detection was done on 20 positive isolates from human, sheep and water samples were confirmed. Toxin is exclusively Enterotoxin (lipopolysaccharide endotoxin) was produced by E. coli isolates from human and sheep fecal samples, as well as water samples. ability to produce two types of virulence factors adhesins that promote binding to specific enterocyte receptors for intestinal colonization and enterotoxins responsible for fluid secretion. survival pathogen due to its antiphagocytic activity and confirm diagnosis of Rfb O157 and fliC H7 gene by PCR assay and gene sequence assay as well as determine antibiotic ability of Escherichia coli.