New Genetic Diversification Of The Type A Pinworm, Enterobius Vermicularis With The Study Of Their Phylogenetic Position And Secondary RNA Structure Analysis

Abstract

aim of this study was to assess the molecular characterization and genotypes of the pinworm, Enterobius vermicularis isolated from children in the city of Erbil, the capital of the Kurdistan Region of Iraq, given the paucity of existing data on the genetic typing of the parasite among Iraqi children. For this purpose, several worms had been collected from infected children in Erbil City then transported to the laboratory. DNA was extracted from the sample. Sequence amplification had been done using universal primer of Cytochrome Oxidase C Subunit One (COI) gene. Results confirm the occurrences of type A genotypes of the pinworm, Enterobius vermicularis. Six local sequences were isolated and recorded in Gen-Bank under the accession number, PV085602, PV085603, PV085604, PV085605, PV085606 and PV085607. Mitochondrial cytochrome c oxidase subunit 1 gene analysis confirmed that the isolated sequences were belong to the type A pinworm, E. vermicularis based on 100% to 99.33% likeness of fractional groupings with type A. Phylogenetic investigation uncovered that the groupings clustered at the side other type C Chimpanzee related E. vermicularis (under the accession number, AB971673 and AB971669) which varies from human pinworm (type A). Results of the secondary structure analysis of the RNA confirms a distinct genetic variation depending on the number and types of their RNA loops. In conclusion, the present study confirms that the isolated sequences are belong to type A human E. vermicularis and it makes a sister group with type C chimpanzee E. vermicularis. A minor genetic diversification among isolated sequences were noticed. The isolation and investigation of genetically diverse type A pinworm, E. vermicularis from human represent the first investigation in Iraq