A in Vitro Study on Picrorhiza Kurroa Root Mediated Synthesis of Silver Nanoparticles and its Anti Inflammatory Activity

Abstract

Introduction: Picrorhiza kurroa is an endangered medicinal herb found in the Himalayas between 3000 and 5000 meters above mean sea level.Due to their multi-target strategy, silver nanoparticles (AgNPs), which may be produced utilizing physical, chemical, or biological (green synthesis) techniques, have displaced all other metal nanoparticles.

Materials And Methods: Silver nanoparticles picrorhiza sample was prepared (picrorhiza + AgNP) sample is prepared and kept in the shaker, Egg albumin and bovine serum albumin assays were done and the anti inflammatory was analyzed.

Results: The sample showed good anti-inflammatory activity when compared to the standard (diclofenac sodium) and this study provides a less toxic, eco-friendly cost effective way of synthesizing and utilization of silver nanoparticles. As the concentration of the sample increases anti inflammatory activity increases which concludes (Pk-root+Ag-Np) sample shows good anti inflammatory activity.

Conclusion: As the concentration increases the anti inflammatory activity increases. It shows better anti-inflammatory activity than already existing standard drugs. Potential anti-inflammatory drugs can be produced using picrorhiza + silver nanoparticles combination after further research in the future.

Introduction: Picrorhiza kurroa is an endangered medicinal herb found in the Himalayas between 3000 and 5000 meters above mean sea level The medicinal properties of P kurroa are attributed to the monoterpenoid picrosides present in the leaves, rhizomes and the roots of the plant However, no genomic information is currently available for P kurroa A total of 710 miRNAs were identified, many of which were found to be associated with molecular responses to temperature changes miRNAs and targets are experimentally validated(Balaji Ganesh, Aravindan, et al., 2025). The availability of the draft genome sequence will facilitate the genetic improvement and conservation of P kurroa The study also provides an efficient way to assemble complex genomes while dealing with duplications when conventional assembly procedures cannot be performed due to duplications.(Sharma et al. 2021)