Evaluation of Toxic Effects of Toothpaste Formation with Moringa Oleifera Purple Bamboo Sea Salt and 9x Bamboo Sea Salt in Animal Model

Abstract

Introduction: The demand for natural health products is rising, with Natural Wellness toothpaste being popular for its benefits. Teeth whitening can be done professionally or at home, addressing extrinsic stains caused by substances like coffee and tobacco. Manufacturers innovate to meet the high demand for effective oral care solutions.

Aim: To develop a natural toothpaste combining Moringa oleifera, purple bamboo sea salt, and 9x bamboo sea salt, aiming to leverage their antibacterial, anti-inflammatory, and mineral-rich properties for enhanced oral health.

Materials and Method:The toothpaste blend includes fluoride for strength, silica for abrasion, glycerin for moisture, sorbitol/xylitol for sweetness, SLS for foaming, xanthan gum for texture, titanium dioxide and hydrogen peroxide for whitening, flavour extracts, preservatives, dicalcium phosphate, sodium saccharin for freshness, and antimicrobial bamboo salts.

Results:This study assessed the antibacterial activity of 9x Bamboo Sea Salt and Moringa Oleifera Purple Bamboo Sea Salt against Lactobacillus sp., S. mutans, and E. faecalis, showing significant inhibition zones. The paste showed no cytotoxic effects on mouse fibroblast cells (3T3-L1) across tested concentrations and exhibited no significant histological changes in liver, spleen, kidney, and lung tissues.Conclusion:Toothpaste with Moringa oleifera Purple Bamboo Sea Salt and 9x Bamboo Sea Salt shows strong antimicrobial properties against S. mutans and Lactobacillus sp., is non-toxic to mouse fibroblast cells, and causes no histopathological changes in major organs. This formulation is a safe and effective alternative to conventional toothpaste.