Development of Linezolid-Graphene Composite-Loaded Nanoengineered Scaffolds for Healing of Burn Wound

Abstract

Linezolid-graphene nanocomposites have enormous potential as an intelligent medication system to efficiently treat burn injuries, resolving important issues such as the requirement for regulated drug release, a high risk of infection, and a prolonged healing period. Here, we created a novel nanoengineered electrospun scaffold for smart burn wound care using graphene composites loaded with Linezolid and embedded in a biocompatible polymeric matrix. Targeted antibacterial activity is provided by the inclusion of Linezolid, a potent broad-spectrum antibiotic, while mechanical strength, thermal stability, and a favorable environment for cell development are provided by the graphene additive. Nanofibers scaffolds with enormous surface area-to-volume ratios, resembling the extracellular matrix, and sustained drug administration were made possible by the electrospinning technique. Both in vitro and in vivo studies demonstrated enhanced fibroblast proliferation and improved re-epithelialization, along with exceptional antibacterial activity against multidrug-resistant infections. Graphene has superior optical, electrical, and thermal capabilities, high surface area of its planar structure, high loading potential, and mechanical and chemical stability; it has been known as a dominant carrier for a wide variety of medicinal compounds. In this work, Linezolid, an antifungal drug, was mingled with Graphene nanoplatets to develop composite via the wet chemical method, embedded in a biocompatible polymeric blend of  Polyvinyl Alcohol (Industrial grade) and subjected to electrospinning to obtain nanoengineered scaffolds (nanofibers) for enhanced antibacterial property. Pursuing a set of optimization experiments, 10% w/v Polyvinyl Alcohol (Industrial grade) was found to be adequate for formation of smooth, defect-free, and regular fibers. Conclusion declared the superior antifungal activity of Linezolid-Graphene entrapped nanofibers as compared to Linezolid entrapped nanofibers and recommended believable dressing materials for a dominant regimen of burn wound infections.