Biochemical and Genetic Study of Muramidase Enzyme in Serum of Patient with Crohn`S Disease with Crohn`S Disease

Abstract

Abnormal immune reactions throughout the digestive tract are a hallmark of Crohn's disease, also known as chronic inflammatory bowel disease. This study looks on the relationship between Crohn's disease and biochemical markers in Mosul City. 140 individuals (70 Crohn's patients and 70 healthy controls) had their serum levels of lysozyme, interferon-gamma (IFN-γ), tumor necrosis factor-α, interleukin-12 (IL-12), interleukin-23 (IL-23) and malondialdehyde (MDA) measured.Additionally, ribonucleic acid (RNA) was extracted using the quantitative polymerase chain reaction (q-PCR) technique from 13 patients and 2 healthy individuals. The results are displayed. The levels of IFN-γ, TNF-α, IL-12, IL-23, MDA, and mur all significantly increased in Crohn's disease patients compared to healthy people. Lysozyme gene expression also increased.