Evaluation Of Antioxidant, Anti-Inflammatory Properties Of Juglans Regia L. And Coffea Canephora L. Combination Extract Capsule- In Vitro Study

Abstract

Background:  Plant-derived bioactive compounds have gained increasing attention as potential therapeutic agents due to their antioxidant and anti-inflammatory properties. Juglans regia L. (walnut) and Coffea canephora L. (robusta coffee) are rich in polyphenols, flavonoids, and other phytoconstituents known to modulate oxidative stress and inflammation. This study evaluated the antioxidant and anti-inflammatory activities of a novel combination extract capsule of J. regia and C. canephora prepared through standardized extraction and encapsulation techniques.

Materials and Methods:  Aqueous extracts of J. regia kernel and C. canephora green beans were homogenized, condensed, lyophilized, and formulated into gelatin capsules containing 500mg of the combination extract. Antioxidant potential was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, while anti-inflammatory activity was determined using the albumin denaturation method. Both assays were conducted at concentrations of 20, 40, 60, 80, and 100 µg/ml, with ascorbic acid and diclofenac sodium serving as standards, respectively.

Results: The extract exhibited a concentration-dependent increase in both antioxidant and anti-inflammatory activities. Maximum antioxidant activity was observed at 100 µg/ml with 69.9% inhibition, compared to 84.8% for ascorbic acid. Similarly, the anti-inflammatory assay showed 74.4% inhibition at 100 µg/ml, which was comparable o diclofenac sodium (87.6%).

Conclusion:  The combination extract of J. regia and C. canephora demonstrated significant in vitro antioxidant and anti-inflammatory activities, supporting its potential role as a natural therapeutic agent for conditions involving oxidative stress and inflammation, including Oral Submucous Fibrosis (OSMF). Further in vivo and clinical studies are warranted to validate these findings.