Fabrication, Optimization And Characterization Of Silver Nanopartices Using Methanolic Leaf Of Claytonia Perfoliata (R.) Nees For Treating Bacterial Infections

Abstract

The ethnomedicinal and phytochemical-rich Claytonia perfoliata (R.) Nees methanolic leaf extract is used to greenly synthesise, optimise, and characterise silver nanoparticles (AgNPs). Methanolic extract was used to reduce and stabilise AgNPs, making biosynthesis an eco-friendly and cost-effective alternative to chemical synthesis. To maximise nanoparticle yield and stability, silver nitrate concentration, pH, temperature, and reaction time were optimised. UV–Vis spectroscopy and SEM examination verified AgNP production and shape. Functional groups responsible for reduction and stabilisation were identified. SEM showed crystalline and spherical nanoparticles. The synthesised AgNPs inhibited a panel of harmful microorganisms, particularly Staphylococcus aureus and Escherichia coli. These findings imply that C. perfoliata methanolic leaf extract can be utilised to greenly synthesize AgNPs with promising bacterial infection treatments, paving the path for plant-based nanotherapeutics.