Preparation of Dental Varnish Using Ziziphus Oenoplia Mediated Selenium Nanoparticles And It’s Antimicrobial Activity Against Oral Pathogens
DOI:
https://doi.org/10.64252/2cfdrn14Keywords:
Dental varnish,selenium nanoparticles,antimicrobial activity,Ziziphus oenoplia,oral microbesAbstract
Background:Nanotechnology plays a major role in the field of dentistry and medicine.Dental caries affect the aesthetics and the quality of life of an individual.The plant extract was used in order to stabilise the antimicrobial activity of the nanoparticles. Creating a dental varnish by integrating nanoparticles with plant extract can be a solution to minimise the side effects of normal dental varnishes.The antimicrobial activity of the varnish was assessed by the Agar well diffusion method.
Methods:The antimicrobial activity of the varnish was assessed by agar well diffusion method by adding to the wells at different concentrations(25, 50, and 100 µg/mL).Time kill kinetic assay was performed to assess the efficacy of the varnish.The standard for bacteria and fungi is Amoxyrite and Fluconazole respect.
Results:It was observed that as the concentration of the varnish increases,the zone of inhibition also increases. The prepared dental varnish exhibits a very strong antimicrobial activity against the oral pathogens like Streptococcus mutans,Staphylococcus aureus,Enterococcus faecalis,Lactobacillus,Candida albicans.
Conclusion:As the prepared dental varnish exhibits excellent antimicrobial activity,further studies can be carried in vivo followed by human trials in order to pave the way for wide usage.
