Anti-Angiogenic Activity of Theaflavin-Coated Copper Nanoparticles Through VEGF Inhibition in Lung Cancer Cells
DOI:
https://doi.org/10.64252/9kn21j82Keywords:
Theaflavin, Theaflavin derived copper nanoparticles, lung cancer, anti-inflammatory activity, VEGF-A, cytotoxicity, A549 CellsAbstract
Background: Lung cancer continues to be the major cause for deaths by cancer, with urgent need for novel therapeutics. Theaflavin, a natural polyphenol, exibits anti-cancer properties, and copper nanoparticles (CuNPs) are known for their redox activity. This study investigates the anti-inflammatory and anticancer efficacy of Theaflavin-derived copper nanoparticles (TheaCuNPs) in lung cancer cells.
Methods: TheaCuNPs were synthesized via green reduction using theaflavin and analyzed by SEM, FTIR, UV-Vis, XRD, and EDX. A549 lung cancer cells were treated with TheaCuNPs, CuNPs, and theaflavin. Cytotoxicity was evaluated by MTT assay, anti-inflammatory activity by protein denaturation and gene expression by qRT-PCR.
Results: TheaCuNPs showed significant dose-dependent cytotoxicity and protein denaturation inhibition, outperforming free theaflavin and CuNPs. qPCR analysis revealed marked downregulation of VEGF-1, HIF-1α, indicating strong anti-antiogenic and anti-metastatic effects.
Conclusion: TheaCuNPs offer promising dual anti-inflammatory and anticancer activity, supporting their potential as a therapeutic candidate for lung cancer.
