In Vitro Controlling of Oral Pathogens Using Commelina Benghalensis Assisted Zinc Oxide Nanoparticles Based Gel

Abstract

Introduction: Many oral disorders, such as cold sores, oral thrush, periodontal disease, and dental caries, are brought on by mouth pathogens, which include viruses, bacteria, fungi, and parasites. The antibacterial action of plant phenolic compounds against human infections has been studied in great detail in an effort to find novel medications, therapeutic goods, and healthy food ingredients. Maintaining a healthy lifestyle, along with routine dental checkups, is crucial to preventing infections. Aim: In order to control oral infections, the purpose of this study is to evaluate the antibacterial activity of a gel based on Commelina benghalensis and zinc oxide nanoparticles (ZnONPs) in vitro. The study focuses on common oral microorganisms in particular. Materials and methods: Mueller Hinton agar was used in the study as the microorganism growing medium. The antibacterial capabilities of zinc oxide nanoparticles (ZnONPs) produced by green synthesis were investigated. Streptococcus mutans, Lactobacillus sp., Staphylococcus aureus, Candida albicans, and Enterococcus faecalis were among the bacterial and fungal strains. As controls, common antibiotics such as fluconazole for fungus and amoxyrite for bacteria were employed. Sterilised petri dishes, cotton swabs, polystyrene tips, an autoclave, and an incubator were necessary lab supplies. Result: For every pathogen examined, the study found discrete inhibitory zones at 25 µg, 50 µg, and 100 µg ZnONPs gel concentrations. The ZnONPs gel supported by Commelina benghalensis showed a noteworthy antibacterial activity, according to the data. Different pathogens had different inhibition zones, with most microorganisms exhibiting dose-dependent suppression. Candida albicans, on the other hand, displayed the maximum zone of inhibition at 100 µg/mL. Conclusion: Strong antibacterial qualities against a variety of oral infections were established by Commelina benghalensis-assisted ZnO NPs gel, indicating its possible use as a therapeutic agent in dental applications