Microbiological Water Quality Assessment of Chambo Based on the Inen 1108 Standard

Abstract

This study aimed to assess the quality of drinking water in the Chambo canton, focusing on the detection of pathogenic bacteria that may pose a risk to public health. A total of 50 water samples were collected from strategic locations throughout the area and analyzed using selective culture media and conventional Polymerase Chain Reaction (PCR), a technique that enables precise molecular identification of microorganisms. Specific primers were used for each target bacterium (Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella spp.), along with positive controls to validate the results. The analysis revealed a concerning presence of pathogens: Listeria monocytogenes was detected in 40% of the samples, Escherichia coli O157:H7 in 80%, and Salmonella spp. in 20%. These findings indicate a significant level of microbiological contamination in the drinking water consumed by the population of Chambo. The purpose of this research is to raise awareness about the health risks associated with contaminated water and to emphasize the urgent need for measures that improve water quality in the area, thereby reducing the population’s exposure to waterborne diseases.