Evaluating The In Vitro Antioxidant And Antimicrobial Properties Of Hybanthus Enneaspermus With GC-MS Profiling

Abstract

Phytochemicals, the chemical substances that occur naturally in plants, are known to demonstrate several biological activities. The current study was performed to assess the phytochemical profile and the antimicrobial and in vitro antioxidant effects of the Hybanthus enneaspermus plant. The leaves and root powder of H. enneaspermus were extracted using an ethanol solvent. The phytochemical profile of the plant was assessed using GC-MS analysis. The occurrence of the diverse functional groups and metabolites in the H. enneaspermus leaves and roots was assessed using FT-IR and 1H NMR analyses, respectively. The capacity for antioxidant activity of the H. enneaspermus leaves was studied by several analytical techniques, including DPPH scavenging, FRAP scavenging, TRAP capacity, ABTS scavenging, NO scavenging, and superoxide radical scavenging assays. The antimicrobial properties of the H. enneaspermus leaves and roots were tested by the disc diffusion technique. The findings of the GC-MS assessment have exhibited the occurrence of various bioactive compounds in the H. enneaspermus leaves and root extracts. The findings of the FT-IR and 1H NMR analyses proved the existence of numerous functional groups and metabolites in the H. enneaspermus leaves and root extracts. The findings of this study also demonstrated potential antimicrobial properties against B. cereus, S. marcescens, and S. aureus. The findings of the antioxidant studies highlighted that the H. enneaspermus leaf extract successfully scavenged DPPH, FRAP, ABTS, NO, and superoxide radicals. The current study proved that the H. enneaspermus extracts exhibited the occurrence of numerous phytochemicals and demonstrated potential antimicrobial and free radical scavenging activities. Hence, it was clear that H. enneaspermus could be beneficial in combating infectious and oxidative stress-mediated diseases.