Toxicity Studies Of Traditional Siddha Polyherbal Formulation Kadukkai Nei In Adult Albino Wistar Rats

Abstract

Introduction: Siddha system is an ancient holistic system of medicine originated from south India now comes under AYUSH becoming popular all over the world. The principle of Siddha system of medicine is “Food is medicine; Medicine is food” that promotes the health of body, mind and soul. Kadukkai Nei an internal Siddha medicine contains eleven herbals and it also includes cow’s milk and ghee. KadukkaiNei is indicated for obesity in classical Siddha text. Though this is a time tested traditional medicine, it lacks scientific validation.Hence it was planned to conduct acute and sub chronic  toxicity studies for Kadukkai Nei in Adult Albino Wistar rats.

AIM: To evaluate Acute and Subchronic toxicity studies for Siddha Polyherbal formulation Kadukkai Nei among Adult Albino Wistar rats. Materials And Methods: In acute toxicity study 3 animals for each step for a total of 2 steps (n= 6 animals) were used. 2000 mg/kg of body weight as starting dose with doses depleting to 300mg, 50mg, and 5mg that were prepared shortly prior to administration .In sub chronic toxicity study10 animals of both the sexes were used in each group. Hence total of 60 animals were used in 3 groups. Kadukkai Nei (KN) in two doses 200mg, 400mg/kg body weight were administered orally. Results And Conclusion: The results of acute toxicity study indicated that Kadukkai Nei via oral route did not produce any signs of toxicity and no mortality were found in animals during the 14 days of study period. Sub chronic toxicity study proven that the drug Kadukkai Nei was well tolerated for about 90days at two dose levels.