Development and Evaluation of Topical Hyalurosomes Nanogel of Calotropis procera for the management of Vitiligo

Abstract

Objective: The analysis and pharmacological assessment of topical hyalurosome nanogel of Calotropis procera for the treatment of vitiligo is the aim of this paper.

Material and methods: Using the B16F10 cell line, cell viability, melanin content, tyrosinase activity assay, and Fontana-Masson silver staining, the anti-vitiligo activity of the nanogel was assessed and carried out. Fresh milky white latex of Calotropis procera was obtained by cutting stem twigs and identified by FT-IR for the presence of active phytoconstituents. Hyalurosomes as nanogel formulation was made using the solvent evaporation method.

Result: The large peak at 3253 cm-1 to the OH-str. frequencies of b-amyrin, lubeol, calotropin, and uscharin was shown by the FT-IR study. Calotropis procera petroleum ether latex contains beta-amyrin, alpha calotropeol, beta-calotropeol, and lupeol. These substances were used as drugs for topical nanogel delivery. In contrast to CP-Carbopol Gel (51.20±9.63%) at 50μM concentration, CPHGF demonstrated 64.57±2.76% cytotoxicity, according to the cell viability assay. The MTT assay reported that at 50 µM concentration, the cytotoxicity of CPHGF was 81.93±11.51% while that of the CP-Carbopol gel was 53.12±11.52%. Tyrosinase activity and melanin revealed that the petroleum latex of Calotropis procera solution increased the amount of melanin by 1.20 times after the first treatment (p<0.05) and again after the second treatment (p<0.05). In the first treatment, CPHGF increased melanin by 2.25 fold (p<0.0001), and in the second treatment, it increased melanin by 2.89 fold (p<0.0001).  To see the melanin pigment in cells exposed to petroleum latex of Calotropis procer a CPHGF formulation, Fontana-Masson silver staining is used.  In petroleum latex of Calotropis procera based nanogel, the quantity of melanin granules was markedly elevated and stained in comparison to the control.

Discussion: The unique deformability of CP and hyalurosomes Additionally, carbopol gel helps improve the skin penetration of latex that contains active ingredients. Higher penetration of the encapsulated moiety through produced CPHGF was confirmed by cell uptake experiments.