Isolation, Identification and Characterization of Secondary Metabolites in Methanol Extract of Stem Bark of Acacia Nilotica

Abstract

Acacia nilotica is commonly known as babul or kikar belongs to the fabaceae family and showed diverse pharmacological actions. Significance of various parts of this plant is mentioned in the traditional medicine system of India. The methanol extract of stem bark of Acacia nilotica was investigated for its phytochemical constituents through chromatographic and spectroscopic techniques. Lupenone, Niloticane, Acanilol-A and Acacetin secondary metabolites were successfully isolated, identified and characterized. These compounds were purified using silica gel column chromatography followed by TLC and structurally elucidated using mass spectrum, FTIR spectrum, ¹HNMR spectrum and ¹³CNMR spectrum data.