Standardization Of Process Of Extraction Of Resveratrol From Underutilized Byproduct Jamun Seed (Syzygium Cumini) Using Ultrasonication

Abstract

Resveratrol is a well-known antioxidant of considerable interest owing to its numerous beneficial health benefits. Jamun seed is an underutilized by-product and is a good source of resveratrol, offering a potential value-added product for these industries. The current investigation aimed to determine the best polyphenol extraction conditions of jamun seed using ultrasound assisted extraction procedure. Response surface methodology was applied to standardize the extraction variables such as process time, solvent concentration, temperature and sonication amplitude to maximize extraction yield. The optimized extraction conditions were 90 minutes, 85% ethanol, 55℃ and 50 Hz. Among the variables analysed for jamun seed extract, total phenolic content was estimated to be 315.92 mg GAE/g while antioxidant capacity as assessed by different assays, namely DPPH, ABTS and FRAP was recorded as 1326.68, 1803.27 & 1174.16 mmol TE/g. The qualitative analysis performed by high performance liquid chromatography demonstrated the presence of resveratrol in the extract.