Evaluation Of Hepatoprotective Potential Of Methanolic Extract Of Achyranthes Aspera Against Isoniazid–Rifampicin-Induced Liver Toxicity In Rats

Abstract

The present study aimed to evaluate the hepatoprotective potential of the methanolic extract of Achyranthes aspera against isoniazid–rifampicin-induced hepatotoxicity in Wistar rats. Phytochemical screening confirmed the presence of flavonoids, phenolics, diterpenes, and tannins compounds known for their antioxidant and liver-protective properties. The extract showed a moderate yield of 7.02% (w/w). Hepatotoxicity was induced by oral administration of isoniazid and rifampicin, resulting in elevated liver enzyme levels (ALT, AST, ALP), increased lipid profile (cholesterol and triglycerides), decreased total protein, and increased bilirubin levels. Oxidative stress markers showed elevated MDA and reduced CAT and GSH levels. Treatment with the methanolic extract of A. aspera at doses of 100 and 200 mg/kg significantly reversed these changes in a dose-dependent manner. The higher dose (200 mg/kg) demonstrated results comparable to the standard hepatoprotective drug silymarin. The findings suggest that A. aspera extract mitigates hepatic damage through antioxidant, anti-lipidemic, and membrane-stabilizing effects, supporting its traditional use for liver-related ailments.