Molecular Identification And Virulence Gene Patterns Of Pseudomonas Aeruginosa Isolates

Authors

  • Khanzad Khudhur Jarjees Author

DOI:

https://doi.org/10.64252/qpkzrr64

Keywords:

Pseudomonas aeruginosa, hemolyytic phospholipase C, elastase, RNA polymerase subunit β gene, multiplex PCR.

Abstract

The current research aimed to assess antibiotic resistance profiles and the detection of virulence factor genes, including lasB, and plcH among the clinical isolates of P. aeruginosa, and to identification of isolates by investigation of the rpoB gene. As well as determine whether a correlation exists between the prevalence of these virulence factors and antibiotic resistance in P. aeruginosa isolates. Pseudomonas aeruginosa infections are associated with significant morbidity and mortality due to the organism’s ability to adapt easily to changes in the environment, rapidly develop resistance to antibiotics, and produce a variety of virulence factors.  P. aeruginosa possesses a large number of virulence factors that may contribute to its pathogenicity, including the P. aeruginosa hemolytic phospholipase C (plcH), which degrades phosphatidylcholine, an abundant lipid in cell membranes and lung surfactant. A zinc metalloprotease called elastase lasB has an elastolytic activity on human tissue, and especially lung tissue. Phylogenetic characterization of the strains most often requires the use of certain household markers or phylogenetic markers, including subunit β RNA polymerase and 16S ribosomal RNA. A total of 50 isolates of P. aeruginosa were supplied from hospitalized burn patients. Identification and antimicrobial susceptibility tests were performed using the VITEK 2 system. Multiplex PCR was done to detect lasB, plcH, and rpoB genes. Multiplex PCR analyses of lasB, plcH, and rpoB genes showed that all P. aeruginosa isolates were positive. The most effective antimicrobial agents against clinical isolates were Meropenem, Amikacin, and Ciprofloxacin. The prevalence of lasB and plcH genes in clinical isolates plays an important role in the development of the disease. Also, phylogenetic markers rpoB used can be exploited for P.  aeruginosa strains of different origin.

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Published

2025-08-04

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Section

Articles

How to Cite

Molecular Identification And Virulence Gene Patterns Of Pseudomonas Aeruginosa Isolates. (2025). International Journal of Environmental Sciences, 1739-1745. https://doi.org/10.64252/qpkzrr64