Environmentally Friendly Exploitation of Endophytic Bacteria from Evolvulus alsinoides and its Bio-molecular Characterization

Abstract

In the microbial world, endophytic microbial communities have come to be a lot of attention in the recent few decades in a variety of sectors of biology. Antimicrobial substances are produced by endophytes in a variety of ways. Endophytes can be a valuable source of bioactive compounds, and they might be recovered, described, and studied in order to find new lead medicinal compounds. The aforementioned study is to spot out endophytic bacteria from Evolvulus alsinoides L. leaves. The plant samples were taken in the Yercaud hill station. Using Tryptic soy Agar, colonies were recovered from surface pre - sterilized leaves of Evolvulus alsinoides L. Screening of Asparaginase, amylase, galactosidase, amino acid and organic acid production by isolates were qualitatively performed. Antimicrobial secondary metabolite extracted from submerged fermentation medium and evaluated by well diffusion method. Antibacterial activity reveals isolate Micrococcus endophyticus, Gram positive Cocci showed antibacterial effect and the extracted metabolite were identified by GCMS. Totally 45 different compounds were identified and Acebutolol, Guanethidine, 2-[2- (Benzoyloxy) ethoxy] ethyl benzoate, Photocitral B and Strychane, 1-acetyl-20.alpha.-hydroxy- 16-methylene are found to be significant compounds and demonstrated a greater capacity for the 3-Oxoacyl ACP reductase enzyme inhibition. In silico antimycobacterial data reveals that these compounds are interacted and acted as novel antimycobacterial agents. By partial 16srRNA sequencing E8 isolate is determined and closely related as Micrococcus endophyticus.