Study the Metabolism of Fatty Acids in Sera of COVID19 Patients in Duhok City/Iraq

Abstract

Background and objectives: COVID-19 has been increasingly reported with derangement of biological pathways which might ultimately affect the metabolism. We sought to identify the impact of COVID-19 on metabolic pathways.

Methods:The study dealt with two groups of people, the first group was (25) men with COVID-19, their ages ranged between (40-60) years, and were undergoing chemotherapy.The second group included (25) healthy men who did not have any apparent disease as a control group, where blood was drawn from the vein area, then the blood serum was separated by a centrifuge, and then the serum was divided into two parts. The first part was used to measure the different biochemical variables that have a Relationship to lipid metabolism of different types in blood serum, while the second part was preserved until analysis of fatty acids.  The serum lipids were extracted using organic solvents, and they were separated from each other using thin-layer chromatography (TLC). Then the percentage of saturated and polyunsaturated fatty acids (mono and poly) was measured using gas chromatography (CGC) for the three fractions of blood serum (CE), (TG), (PL) after the fatty acid re-esterification process. Results: It was observed that there are differences between the percentage of fatty acids of various types between the group of patients and the control group and in the different parts of the blood serum lipids, and this indicates the effect of fatty acids in the development of COVID-19  diseases, including risk factors for cardiovascular disease through the difference and imbalance that occurs in the percentage, especially of poly-saturated fatty acids (PUFA). Conclusion: The derangement in metabolic parameters have been confirmed and taken to consideration for future direction of treatment and challenges of viral infections.