The Effect Of PRP (Platelet Rich Plasma) Administration On The Histomorphometric Profile Of The Rabbit Ovarian Follicles

Abstract

The ovaries of Oryctolagus cuniculus rabbit are small, ovoid organs situated laterally in the pelvic cavity, comprising an outer cortex and inner medulla containing follicles and stromal tissue, giving their essential role in oocyte production and hormonal regulation, enhancing ovarian function. Platelet-rich plasma (PRP), has clinical significance increasingly utilized across medical disciplines, has emerged as a potential therapy to improve reproductive outcomes. This quantitative analysis study, conducted at the Department of Human Anatomy, College of Medicine, Al-Nahrain University started in (Oct 2024), to evaluate PRP’s anatomical and histological effects on rabbit ovaries. Sixty female rabbits were divided into three groups: Group A (control), Group B (intraperitoneal PRP), and Group C (intravenous PRP). Histological analysis revealed significant increase number of primordial and mature follicles, as well as thicker granulosa and thecal layers in both PRP-treated groups compared to controls (p < 0.001). Notably, the intraperitoneal group showed superior improvements in folliculogenesis and vascularization relative to the intravenous group. Differences in theca interna thickness between PRP groups were also significantly different (p < 0.001). These findings underscore PRP's efficacy—particularly via the intraperitoneal route—in enhancing ovarian structure and function, suggesting its potential as a therapeutic approach for fertility preservation and ovarian rejuvenation