Eco Friendly Analytical Method Development And Validation Of Clevidipine By Using Reverse Phase Ultra Performance Liquid Chromatography

Abstract

A simple, Accurate, precise, Ecofriendly analytical method was developed for the estimation of the Clevidipine in Api. Chromatogram was run through ACQUITY UPLC CHS C18 Column, 100x 2.1mm 1.8mm. Mobile phase containing Buffer KH2PO4: Acetonitrile taken in the ratio 60:40 was pumped through column at a flow rate of 0.3 ml/min.. Temperature was maintained at 30°C. Optimized wavelength selected was 240.0 nm. Retention time of Clevidipine was 1.146. Standard %RSD of Clevidipine were and found to be 0.7. %Recovery was obtained as 99.60%. LOD, LOQ values obtained from regression equations were 0.01, 0.03. Regression equation of Clevidipine is y = 51673x + 2873.4.Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.