Wound Healing Activity Of Different Extracts Of Some Indian Medicinal Plants In Some Animal Models

Abstract

The advance oxidation protein products are markers of oxidative stress and protein glycation and mediators of oxidation of super oxide and plasma proteins.  AOPP and neopterin are responsible for delayed macrophage activation and phagocytosis, which are the part of first phase of wound healing.The ethanolic and petroleum ether extracts were evaluated for wound healing activity in normal rats. The ethanolic extract showed highest significant wound healing activity than the other extracts by promotion of various biochemical factors in non treated rats. In wound healing study of ethanolic extract; the excision wound model was created to study the healing of wounds in terms of epithelialization, wound area and wound closure measurement. The ethanolic extract showed faster scar falling and epithelialization as compare to control non -treated normal rats. The Epithelialization represents the completion of remodelling and maturation phase and is the sign of complete healing of wound. The decrease in wound area resembles increased % of wound closure in treated rats. The faster wound closure is occurred only in condition of rapid proliferation of granuloma tissues.

In dead space wound healing model we studied inflammatory and proliferation phase of wound healing. The weight of wet granulation tissue express the inflammatory phase while the weight of dry granulation tissue with hydroxyproline, collagen and elastin represents the proliferation of granuloma tissue. The ethanolic extract treated rats showed increased weight of wet and dry granulation tissue at site of wound.