Assessment Of Antioxidant Potential And Structural Characterization Of Hemicelluloses Extracted From R. Raetam Roots And Stems Via FTIR Spectroscopy

Authors

  • Zohra KEBIR Author
  • Aicha BOUHAFSOUN Author
  • Abdellah MOUSSAOUI Author

DOI:

https://doi.org/10.64252/se64b168

Keywords:

Antioxidant activity, hemicelluloses, R. raetam, DPPH, IC₅₀, inhibition kinetics, FTIR, acetylated xylans.

Abstract

This study evaluates the antioxidant activity of hemicelluloses (HC) extracted from the roots and stems of Retama raetam, a medicinal legume native to the Saharan region. Samples were collected in southern Algeria and subjected to alkaline extraction of polysaccharides. Antioxidant activity was assessed using the DPPH assay, measuring the ability of the extracts to scavenge free radicals. The results showed dose- and time-dependent inhibition, with percentages ranging from 8.97% to 81.22%.

IC₅₀ values were determined using the Hill sigmoid model via the curve-fit function (SciPy library, Python 3.10), executed on the Kaggle platform. The obtained values were 6.54 mg/mL for roots, 24 mg/mL for stems, and 0.37 µg/mL for ascorbic acid (used as a reference antioxidant). Root extracts exhibited stronger antioxidant activity, although their action was slower than that of the stem extracts.

Kinetic analysis revealed a biphasic response, characterized by an initial rapid phase followed by a plateau between 72 and 96 hours. At 40 mg/mL, the roots reached a maximum inhibition of 81.22% after 96 hours, compared to 50.88% for the stems at 72 hours. At lower doses, the roots maintained notable effectiveness.

FTIR analysis of the HC in solid state revealed characteristic bands corresponding to OH, C–H, and C=O groups. After solubilization in NaCl (5 mM), attenuation of these bands indicated weakened hydrogen bonding and conformational reorganization of acetylated xylans, promoting greater chain flexibility.

These findings confirm the promising antioxidant potential of R. raetam hemicelluloses, particularly those extracted from the roots.

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Published

2025-07-02

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Section

Articles

How to Cite

Assessment Of Antioxidant Potential And Structural Characterization Of Hemicelluloses Extracted From R. Raetam Roots And Stems Via FTIR Spectroscopy. (2025). International Journal of Environmental Sciences, 1119-1128. https://doi.org/10.64252/se64b168