Analysis Of Blashv Gene Sequencing In UTI-Associated Klebsiella Pneumoniae Isolates

Abstract

Particularly in urinary tract infections (UTIs), Klebsiella pneumoniae, which produces extended-spectrum beta-lactamases (ESBLs), is become more resistant to various cephalosporin generations. Finding differences in the blaSHV genes of the local isolates of uropathogenic K. pneumoniae was the goal of this investigation. The PCR results for 20 specific blaSHV genes of the isolates that produce ESPL and are resistant to all beta-lactam antibiotics utilized except Meropenem and Imipenem were selected for sequencing study. The blaSHV genes were correctly identified by PCR when the acquired sequences (19) were aligned with the reference strains in GenBank. Additionally, these sequences were examined for the presence of gene variations and nucleotide differences with the reference sequence of SHV-1. The results also indicated that some isolates had the variant SHV-11. The results of detection of amino acid alterations in the products of blaSHV genes revealed that the most amino acid substitution was Arginine to Lysine (R→K) at the positions 70, 132 and 216. Another amino acid substitution was Ileucine to Methionine (I→M) at the position 24 in the isolates (RK12, RK13, RK15, and RK16). Also, changing at the position 71 by presence Proline instead of Arginine (R→P) in the isolates (RK8, RK9, RK10, RK13 and RK15). It was obvious that the isolate RK16 had 5 amino acid substitutions; three of them were Arginine to Lysine. The results confirmed that the most amino acids substitutions were detected in new positions not recorded in the previous studies.