Phytochemical Analysis, Identification Of Bioactive Compounds Using FTIR, GC-MS And Evaluation Of Antioxidant Potential From (Hoom) Miliusa Tomentosa

Abstract

Plant derived biologically active compounds have gained importance in food and pharmaceutical industries. The present study aimed to is to explore the bioactive compounds and their antioxidant and antimicrobial properties in the crude extract of Miliusa tomentos. To identify the active components, the extract was deconvoluted using a successive extraction process with hexane, ethyl acetate and chloroform to isolate the active fraction. The chloroform extract demonstrated the highest inhibitory activity and was selected for further phytochemical investigations. Further, the crude extract was subjected column FTIR and GC-MS to determine the bioactive compounds. γ-Sitosterol, 1-Heptatriacotanol, 4 hydroxyl phenylacetic acid 7-Tetradecenal, n-Hexadecanoic acids were identified as potential compounds. The extracts were also assessed for their antioxidant activity using DPPH, ABTS and FRAP assays, it is concluded that the phytochemicals were revealed that Miliusa tomentosa is highly valuable in medicinal usage for the treatment of various human aliments.