In-Vitro Propagation Of Grapes Cv. Flame Seedless

Abstract

The present investigation entitled “In-Vitro Propagation of Grapes cv. Flame seedless” were carried out in the biotechnology laboratory and experimental field of Horticulture, faculty of agriculture, Guru Kashi University, Bathinda. Out of different sterilizing treatments the highest survival rate (60.00%) and lowest contamination percentage (40.00%) were observed in treatment containing Bavistin (0.3%) + Ethanol (70%) +HgCl₂ (0.1% for 3 min). For shoot proliferation the maximum shooting percentage (94.00%), highest number of shoots/explants (4.75) and maximum length of shoot/explant (3.75cm) were observed in MS media fortified with BA 2.0mg/l + KIN 0.1mg/l. The Maximum rooting percentage (80.00%), highest number of roots (4.25%) and maximum length of root (6.75 cm) were significantly obtained from treatment fortified with MS Media+ IBA 2.0 mg/l.