Bacteriological Profile And Antimicrobial Susceptibility Pattern Of Bacteria Isolated From Pus/Wound Swab Sample From Diabetic Patient Attending Tertiary Care Hospital, Chengalpattu District

Abstract

Background: Diabetic foot ulcers (DFUs) commonly result in days of significant hospital stays and are the primary cause of lower limb amputations in medicine. To choose the best antibiotic treatment, it is essential to identify the bacteria causing DFUs. Numerous studies have shown that antibiotic resistance has dramatically increased, particularly in wound infections in diabetic patients.
Objectives: In order to determine the prevalence of bacteria and the profiles of their antibiotic sensitivity, the current study looked at a variety of patient cultures with DFUs.
Methods: This is a cross-sectional study. 253 DFU patients who were attending the OPD of a tertiary care hospital at Chengalpattu district, Tamil Nadu, India was included in this study. Sterile swabs were used tocollect the samples needed for testing. Routine microbiological laboratory Conventional tests were done and results were recorded.
Results: A total of 155 microorganisms were isolated. Klebsiella spp (36.13%), Escherichia coli (19.35%) and Proteus spp., (9.68%) were the major three organisms isolated. The most often isolated gram-positive and gram-negative bacteria were Staphylococcus species and Klebsiella pneumoniae, respectively. Vancomycin shown 100% sensitivity to all Gram-positive bacteria, although meropenem and Piperacillin/tazobactum werefound to be effective against Gram negative bacteria.
Conclusion: In conclusion, because multiple bacteria may be implicated and multidrug-resistant strains may evolve, doctors are encouraged to take cultures into consideration before beginning empirical therapy.