Stability Indicating Method Development For Simultaneous Estimation Of Carvedilol (Cdl) And Ivabradine (Ivd) By Hplc

Abstract

A simple, accurate, and precise RP-HPLC method was developed and validated for the simultaneous estimation of Carvedilol (CDL) and Ivabradine (IVD) in bulk and pharmaceutical dosage forms. The chromatographic separation was achieved using a C18 column with a mobile phase consisting of a suitable buffer and acetonitrile in an optimized ratio. The method exhibited excellent linearity in the concentration range of 1–5 µg/ml for both CDL and IVD with correlation coefficients (r²) of 0.9996 for each drug. System suitability parameters, such as retention time, tailing factor, and number of theoretical plates, were within acceptable limits, ensuring efficient system performance. The recovery study confirmed the accuracy of the method, with percent recoveries ranging between 97.13% and 98.72% for both drugs. The method also demonstrated excellent repeatability, day-to-day precision, analyst-to-analyst reproducibility, and robustness, with %RSD values consistently below 1.5%. Furthermore, forced degradation studies confirmed the stability-indicating nature of the method. This validated method can be effectively applied for routine quality control and stability testing of CDL and IVD in pharmaceutical formulations.