Ameliorative Potential Of Alcoholic Ginseng Extract On Ibuprofen-Induced Reproductive Toxicity In Male Rats

Abstract

The therapeutic benefits of ginseng (Panax ginseng) have attracted an abundance of interest. So, this investigation was aimed to study the effect of alcoholic Ginseng extract against Ibuprofen induced reproductive dysfunction in male rat. This investigation was carried out from August 24 to September 26, 2024, in the animal house of Veterinary Medicine College at Tikrit University. Seventy (70) adult, seemingly healthy Albino male rats were acquired from the Veterinary Medicine College's animal house at Tikrit University. The results showed that the Testosterone and FSH concentrations were normal in all groups and there were no significant differences (P≤0.05) between groups.On the other hand, significant (P≤0.05) differences were found in the concentration of LH, where the highest concentration was in the fourth group, which took Ibuprofen for 30 days (15.493 ± 0.591) compared to the control group (14.556 ± 1.008), while the treated groups did not show significant differences (P≤0.05) compared to the control group. Sperm motility, sperm life, sperm dead, abnormal sperm and Sperm counts in Ibuprofen (30 days) group and Ibuprofen (10 days) group showed significant (P≤0.05) differences compared to the control group. In the treated groups and ginseng group showed non-significant (P≤0.05) differences compared to the control group. Coenzyme Q10 activity in Ibuprofen (10 days) group showed significant (P≤0.05) elevated compared to the control group. while, in the treated groups and ginseng group showed non-significant (P≤0.05) differences compared to the control group. The Carnitine levels in all groups showed non-significant (P≤0.05) differences compared to the control group. Histological examination of rats dosed with both dimethyl sulfoxide and ibuprofen for 10 and 30 days revealed some histological changes, including hemolyzed blood vessels, reduced spermatogonia, and decreased sperm count. However, after using ginseng extract to treat and prevent the harmful effects of ibuprofen, it was found that seminiferous tubules were containing the different stages of spermatogenic development, and most spermatogenic cells were improved. Its concluded that the alcoholic extract of ginseng roots shows a protective and therapeutic effect on sex hormones and spermatogenesis cells, and improves spermatogenesis.