Detection Of Aph And Tlr C Genes In Local Isolate Of Streptomyces Fradiae

Abstract

Background: Streptomyces is a genus of Actinobacteria, which are Gram-positive bacteria that generate spores and have a filamentous structure. Streptomyces fradiae belongs to the Actinomycetota species. Various variants of S. fradiae have been identified as producers of the antibiotics neomycin, tylosin, and fosfomycin. Aim of the study: Isolation and identification of Streptomyces fradiae and detection of aph and tlrC genes in the isolates. Methods: A total of fifty soil samples were taken between December 2023 and January 2024. Specimens were gathered from several locations inside the city of Baghdad. After sample dilution it put on ISP2 agar for Streptomyces isolation. Several biochemical tests were done for the isolates. Aph and tlrC genes were detected by PCR. Results Thirty Streptomyces isolate were obtained depended on morphological, cultural and biochemical characteristic. The colonies under suspicion were cultivated on ISP2 agar and chosen based on their color (gray, creamy, or white) and colony diameter (ranging from 1 to 10 mm). Additionally, their morphology was considered, with the colonies initially having a smooth surface and later becoming powdery, soft, and granular as they formed aerial mycelium. One isolate only was contain the aph and tlrC genes among thirteen Streptomyces isolates. Conclusion: Actinomycetes were observed in  culturing the diluted soil sample (10^-9) for 7-10 days on ISP2 agar. The PCR amplification exhibited that the aph and tlrC genes was appeared in the one isolated and purified Streptomyces fradiae that associated with resistance.