Formulation, Characterization, And Assessment Of Nanoparticles Incorporating Lyophilized Amla Pulp For Antidiabetic Efficacy

Abstract

This study explores the green synthesis of silver nanoparticles (AgNPs) using Phyllanthus emblica (Amla) fruit extract, renowned for its medicinal properties. The synthesis process involved reducing silver ions with phytochemicals present in the extract, resulting in spherical AgNPs confirmed through UV-vis spectroscopy, FTIR, and SEM analyses. Characterization revealed successful formation of nano-sized particles stabilized by bioactive compounds. The antidiabetic potential of these biosynthesized AgNPs was evaluated in vitro by assessing their inhibitory effects on key carbohydrate-digesting enzymes, α-amylase and α-glucosidase. Results demonstrated significant enzyme inhibition, with the synthesized nanoparticles showing comparable or superior activity to standard drugs like Acarbose. These results indicate that environmentally synthesized silver nanoparticles derived from Phyllanthus emblica may have significant potential as therapeutic agents for diabetes treatment, highlighting the need for additional research to understand their mechanisms of action and evaluate their effectiveness in clinical applications.