Enhancement Of Phytochemical Activity In Orange Juice Using Giloy (Tinospora Cordifolia)

Abstract

The present investigation was carried out to develop and standardize value added orange juice  by incorporating giloy (Tinospora cordifolia) leaves extract at varying concentrations of 10, 15 and 20 per cent. Tinospora cordifolia, a medicinal plant of the Menispermaceae family, is well known for its potent bioactive compounds and significant therapeutic applications, including immunomodulatory, anti-inflammatory, and antioxidant activities. Phytochemical evaluation revealed a statistically significant (p<0.05) increase in alkaloids, flavonoids, tannins, and steroids with higher levels of giloy supplementation. DPPH radical scavenging assay demonstrated a progressive enhancement in antioxidant capacity across all supplemented formulations, with the highest value recorded in Type-III. However, sensory analysis using a 9-point hedonic scale indicated that increasing concentrations of giloy adversely affected organoleptic attributes such as taste, aroma, texture, and overall acceptability. Type-I (10% GLP) exhibited the most favorable sensory profile while also offering improved phytochemical and antioxidant benefits over the control.The study concludes that supplementation of orange juice with 10% giloy leaf extract represents an optimal formulation that balances enhanced nutritional and functional properties with acceptable sensory quality. This formulation has potential for commercialization as a health-oriented functional beverage that meets consumer preferences and supports dietary interventions for improved health and wellness.