Cytotoxicity Effect Of Combined Silver And Zinc Oxide Nanoparticles (Agnps + Zno Nps) On Phagocytic And Cytotoxic Activity Of Macrophages To Cutaneous Leishmania Parasite In Vitro

Abstract

This study evaluated the ability of zinc nanoparticles (ZnO NPs) and silver nanoparticles (AgNPs) combined to stimulate macrophages to produce reactive oxygen species (ROS) capable of destroying  Leishmania tropica parasites. This study used quantitative bioassays via MTT assays. The primary objective was to explore the efficacy of the nanoparticles. .In order to assess the possible additive or synergistic effects of mixing these nanoparticles, four duplicates of each concentration (0.5, 1.0, and 2.0 µg/ml) were employed.  Statistical validation used the least significant difference (LSD) to validate the test results, revealing significant differences between treatments, clearly demonstrating their significant antiparasitic efficacy